EMBOSS explorer

est2genome

Align EST and genomic DNA sequences (read the manual)

Unshaded fields are optional and can safely be ignored. (hide optional fields)

Input section

Spliced EST nucleotide sequence(s). Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:

Unspliced genomic nucleotide sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:
Additional section
Score for matching two bases
Cost for mismatching two bases
Gap penalty
Intron penalty
Splice site penalty
Minimum accepted score
Advanced section
Reverse orientation?
Use donor and acceptor splice sites?
Comparison mode
Print out only best alignment?
Space threshold (in megabytes)
Shuffle
Random number seed
Output section
Show the alignment?
Alignment width
Run section
Email address: If you are submitting a long job and would like to be informed by email when it finishes, enter your email address here.